Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome

Figure 1

In vitro study showed SW therapy enhanced mitochondrial transfusion into the rat lung epithelial cells. (A1 to A3) Illustrating the DAPI stain (400x) for identification of rat lung epithelial cells in three groups (i.e., SC, Mito, and Mito + SW). (B1 to B3) Showing MitoTracker stain (400x) for identification of endogenous mitochondria (green color) among three groups. (C1 to C3) Indicating the MitoTracker stain (400x) for identification of exogenous mitochondria to be transfused into the epithelial cells (red color). (D1 to D3) Indicating the merged pictures of B1–B3 and C1–C3. Pink-yellow color indicated that the endogenous and exogenous mitochondria colocalized together. An abundant number of mitochondria were found in the Mito + SW group. (b) Analytic result of mitochondrial level in the cells, versus other groups with different symbols (†, ‡), . All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( for each group). Symbols (, †, ‡) indicate significance (at 0.05 level). SC = sham control; Mito = mitochondria; SW = shock wave.

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