Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study

Table 3

The effects LPS, GnRH, and LBP on the relative gene expression of TLR4 and proinflammatory cytokines in the AP explants.

Gene	Animals	Anterior pituitary explants
		Control	GnRH	LPS	LPS + GnRH	LPS + LBP	LPS + LBP + GnRH
TLR4	Saline treated	1 ± 0.1^A	0.9 ± 0.2^A	1 ± 0.1^A	0.9 ± 0.1^A	1 ± 0.1^A	0.8 ± 0.1^A
	LPS treated	1.1 ± 1.5^A	1.4 ± 2.2^A	1.2 ± 2.8^A	1.3 ± 1.9^A	1.1 ± 1.2^A	1.3 ± 5^A
IL1B	Saline treated	2.1 ± 0.3^A	2.5 ± 0.3^A	2.3 ± 0.2^A	2.6 ± 0.5^A	1.7 ± 0.3^A	2.6 ± 0.4^A
	LPS treated	2.3 ± 0.4^A	2.5 ± 0.2^A	2.9 ± 0.2^A	2.7 ± 0.2^A	2.9 ± 0.2^A	2.9 ± 0.2^A
IL-6	Saline treated	7.4 ± 0.9^A	7.1 ± 1.2^A	9.3 ± 0.5^A	8.1 ± 0.7^A	13.9 ± 1.6^B	13.8 ± 1.7^B
	LPS treated	18.4 ± 1.5^A	17.3 ± 2.2^A	33.5 ± 2.8^CD	24.9 ± 1.9^B	27.1 ± 1.2^BC	36.1 ± 5^D
TNF	Saline treated	1 ± 0.2^A	0.9 ± 0.3^A	1.4 ± 0.2^A	1.5 ± 0.3^A	1.1 ± 0.1^A	1.1 ± 0.2^A
	LPS treated	1.1 ± 0.2^A	1.2 ± 0.1^A	1.4 ± 0.1^A	1.3 ± 0.1^A	1.2 ± 0.1^A	1.2 ± 0.1^A

All data are presented as the mean (± S.E.M.). Different capital letters indicate significant () differences according to repeated-measures three-way analysis of variance (ANOVA, GraphPad Prism, San Diego, CA, USA) followed by a post hoc Sidak’s multiple comparison test. Gene expression data were presented as normalized to the control of saline-treated group.