Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34⁺ Cells

Figure 5

Survival of CB- and mPB-derived CD34⁺ cells after cocultures with HD-BMSCs and in the presence or the absence of combined inflammatory cytokines. (a) Comparison between 24-hour monocultures and cocultures of CB and mPB-derived CD34⁺ cells ( independent experiments, resp.) with BMSCs for in vitro survival (Annexin V/PI staining) is shown. Percentages of live CB- or mPB-CD34⁺ cells in the presence of BMSCs and/or proinflammatory cytokines with two by two combination (b) or multiple combinations (c) in comparison to CD34⁺ cells cocultured with BMSCs but without inflammatory stimuli are shown. For each graph, to highlight the comparison with cocultures, a dot line represented the mean percentage of live cells in all monocultures (CB and mPB CD34⁺ cells) ( and versus control cells) ( CB vs mPB).

(a)

(b)

(c)