Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation

Figure 3

Effect of APO on ASC translocation during inflammasome activation. LPS-primed BMDMs were pretreated with APO or KCl (150 mM), and IL-1β secretion was determined by Western blotting upon stimulation with (a) 5 mM ATP, 10 μM nigericin for 1 h, and (b) 150 μg/mL silica for 3 h in the supernatant (Sup). NLRP3 and ASC distribution was estimated by Western blotting of the soluble and insoluble fractions of Triton X-100. β-Actin was used as an internal control in each fraction. LPS, lipopolysaccharide; BMDMs, bone marrow-derived macrophages; APO, A. princeps extract; IL, interleukin; Nig, nigericin.

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