Figure 5: Decreased MMP in the sperm of ORN-treated rats. (a) Immunofluorescence of mitochondria in rat sperm, shown by MT-DR FM. Mitochondria in the midpiece of sperm were dyed red, and sperm nuclei were stained with Hoechst 33342 (blue). (b) Histogram showing the percentage of MT-DR FM-positive sperm. Note that the sperm in ORN-treated rats showed a significant reduction in MMP. , one-way ANOVA test, rats in each group. Scale bar = 50 μm. MT-DR FM: MitoTracker Deep Red FM.