Figure 6: Decreased CI enzyme activity in the testes and sperm of ORN-treated rats. (a) Analysis of the CI activity of sperm in control and ORN-treated rats determined by CI enzyme activity microplate assay kit. CI enzyme activity in sperm was expressed in mOD/min. The histogram shows the ratio of the normalized mOD/min of rats in the ORN group compared to that in the control group. Note that CI activity in AS rat sperm was reduced relative to that in the control subjects. (b) Analysis of CI activity in testes of rats in the control and AS groups. The data indicate that CI activity in the testes of ORN-treated rats decreased significantly compared to that in the control group. , , one-way ANOVA test, rats in each group. CI: complex I.