(a)
(b)
Figure 3: Expression levels of cytokine and iNOS mRNA by macrophages infected with L. (L.) amazonensis and treated with hydroalcoholic extract of C. brasiliense. (a) L.L.a: L. (L.) amazonensis; C.b.: C. brasiliense. L.L.a + C.b.: peritoneal macrophages from BALB/c mice were infected with promastigotes of L. (L.) amazonensis (10 parasites: 1 macrophage) for 3 h and then treated with hydroalcoholic extract of C. brasiliense (30 μg/ml) for 3 h; L.L.a.: macrophages infected and untreated; control: macrophages uninfected and untreated were incubated only with RPMI during 6 h (negative control). significantly compared to negative control; compared to infected and untreated macrophages. (b) Cytokine mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) DNA fragments was separated in 1.5% agarose gel electrophoresis and revealed with ethidium bromide in a transilluminator. M: molecular marker; C−: amplification negative control (H2O); C+: DNA obtained from macrophages stimulated with 5 μg/mL of LPS (positive control); 1 and MO: macrophages (negative control, uninfected and untreated); 2 and L.L.a. + C.b.: macrophages infected with L. (L.) amazonensis and treated with C. brasiliense; 3 and L.L.a.: macrophages infected with L. (L.) amazonensis.