Table 1: Gene and PCR conditions.

GeneReferenceAnnealing temperature (°C)CyclesFragments (bp)

TNF-αKolodziej et al. [31]6136276
IL-1βChen et al. [32]5730204
IL-12Kolodziej et al. [31]6136312
IL-18Kolodziej et al. [31]6136440
IFN-γChen et al. [32]5635336
iNOSChen et al. [32]5530311
IL-10Kolodziej et al. [31]6136256
GAPDHByrne et al. [33]6035239

GAPDH: glyceraldehyde-3 phosphate dehydrogenase; IFN-γ: interferon-γ; IL: interleukin; iNOS: inducible nitric oxide synthase; TNF-α: tumour necrosis factor α.