The Effect and Mechanism of TLR9/KLF4 in FFA-Induced Adipocyte Inflammation

Figure 3

The regulatory effect of KLF4 on downstream inflammatory factors. (a) The differentiation of 3T3-L1 adipocytes; (b) the mRNA and protein expression level of KLF4 after KLF4 interference for 24 h and 48 h; (c) detecting the protein expression level of p-P65 after downregulation of KLF4; (d) detecting the mRNA expression levels of P65, IL-6, MCP-1, TNF-α, and APN after downregulation of KLF4; (e) release levels of IL-6, MCP-1, TNF-α, and APN after downregulation of KLF4; (f) mRNA and protein expression levels of KLF4 after pIRES2-KLF4 plasmid was transfected into mature adipocytes after 24 h and 48 h; (g) detecting the protein expression level of p-P65 after upregulation of KLF4; (h) mRNA expression levels of P65, IL-6, MCP-1, TNF-α, APN, and IL-1β; (i) detecting the release levels of IL-6, MCP-1, TNF-α, and APN after upregulation of KLF4. -test, , ; the difference has a statistical significance. The effects on KLF4 expression after upregulation of TLR9.

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