Pharmacological Inhibition of Caspase-1 Ameliorates Cisplatin-Induced Nephrotoxicity through Suppression of Apoptosis, Oxidative Stress, and Inflammation in Mice

Figure 4

Effects of Ac-YVAD-cmk on cisplatin-induced oxidative damage in the kidneys. Representative images of immunohistochemical staining using anti-4-HNE antibody in the cortex (a) and glomerulus (b). Scale bar: 25 μm. Graphs show the percentage of 4-HNE-stained area per field in the cortex (c) and glomerulus (d). (e) Level of malondialdehyde (MDA). (f) GSH/GSSG ratio. Real-time RT-PCR analysis of CYP2E1 (g), SOD2 (h), catalase (i), and glutathione synthetase (j) in the kidneys. Con: control, CP: cisplatin, and CP + YVAD: cisplatin plus Ac-YVAD-cmk. per group. All data are expressed as the mean ± SEM. and vs. Con. and vs. CP.

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