Apoptosis-Associated Speck-Like Protein Containing a CARD Deletion Ameliorates Unilateral Ureteral Obstruction Induced Renal Fibrosis and Endoplasmic Reticulum Stress in Mice
ASC deletion reduces the expression of cytokines that are upregulated by inflammasomes in the UUO model of CKD. (a) Immunoblotting for the level of caspase 1, IL-18, and IL-1β. (b) Quantification analysis of caspase 1, IL-18, and IL-1β levels, normalized against those of β-actin. Semiquantitative analysis of NLRP3, IL-18, and IL-1β (c) and IL-6 and TNF-α gene expression (d) normalized against 18S performed by real-time PCR. , WT/sham group versus WT/UUO group. , ASC−/−/UUO group versus WT/UUO group. Data represent the mean ± SEM ().