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Figure 5: Icaritin is the active ingredient that improves the abnormal proliferation of megakaryocytes and invokes the TPO/MPL axis in the bone marrow of ITP mice. (a) HE staining of the bone marrow. Black arrows indicate megakaryocytes. (b) Icaritin decreased the number of megakaryocytes in the bone marrow of ITP mice. (c) TPO immunohistochemical staining in the bone marrow. (d) Icaritin inhibited TPO protein expression. (e) MPL immunohistochemical staining in the bone marrow. (f) Icaritin decreased MPL protein expression in megakaryocytes. (g) p-Stat3 immunohistochemical staining in the bone marrow. (h) Icaritin inhibited p-Stat3 protein expression in megakaryocytes. Data are presented as the mean ± SEM ( per group). Scale bar = 50 μm, ×200. One-way ANOVA was used to analyze the difference among the groups. and versus control group; , , and versus model group.