Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats

Figure 1

LA inhibited the mRNA levels of LPS-induced proinflammatory mediators and inhibited the adhesion of monocytes to HUVECs. The molecular structure of LA (a). HUVECs were pretreated with 50 μM of LA for 60 min before treatment with LPS (100 ng/mL) (b) for 6 h. Expression of IL-1β, TNF-α, IL-32β, MCP-1, CCL-2, and VCAM-1 mRNA levels were measured using RT-PCR. HUVECs were pretreated with a blocking mouse antihuman monoclonal antibody (E1/6) against VCAM-1 or various doses of LA for 60 min before treatment with LPS. U937 mononuclear cells adhered to LPS-stimulated HUVECs were observed through microscopic images (c). Data represent the of at least three independent experiments, and each experiment was performed in triplicate. versus control; versus LPS; versus LPS; versus LPS.

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