Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats

Table 1

Quantitative RT-PCR primer sequences.

Gene	Direction	Primer
TNF-α	Forward	CAGAGGGAAGAGTTCCCCAG
	Reverse	CCTTGGTCTGGTAGGAGACG
IL-1β	Forward	AAACAGATGAAGTGCTCCTTCCAGG
	Reverse	TGGAGAACACCACTTGTTGCTCCA
VCAM-1	Forward	TTGCTCAGATTGGTGACTCCGTCT
	Reverse	TTCGTCACCTTCCCATTCAGTGGA
NLRP3	Forward	TTCGGAGATTGTGGTTGGG
	Reverse	CAGGTAAAGGTGCGTGAGATT
IL-32β	Forward	GAGTTTCTGCTGCTCTCTGTCA
	Reverse	ATTTTGAGGATTGGGGTTCAG
eNOS	Forward	TCACCGCTACAACATCCT
	Reverse	CCTTCTGCTCATTCTCCA
GAPDH	Forward	AGAAGGCTGGGGCTCATTTG
	Reverse	AGGGGCCATCCACAGTCTTC
NLRP3 (rat)	Forward	TGAAGAGTGTGATCTGCGGAAAC
	Reverse	GAAAGTCATGTGGCTGAAGCTGT
GAPDH (rat)	Forward	CAACGGGAAACCCATCACCA
	Reverse	ACGCCAGTAGACTCCACGACAT