The Kat in the HAT: The Histone Acetyl Transferase Kat6b (MYST4) Is Downregulated in Murine Macrophages in Response to LPS

Figure 5

Analysis of the role of the miR-487b binding site in the 3UTR of the Kat6b mRNA. The Kat6b 3UTR was cloned downstream of the luciferase open reading frame in the pLightswitch-3UTR reporter plasmid (pKat6bLuc-3UTR). A second plasmid was prepared with the miR-487b binding site deleted (pKat6bLucΔ3UTR). RAW264.7 macrophages were transfected with either pKat6bLuc-3UTR or pKat6bLucΔ3UTR. Transfected cells were then treated with LPS for 6 hr (100 ng/ml) and analyzed for luciferase expression (). RAW264.7 cells were also cotransfected with either pKat6bLuc-3UTR or pKat6bLucΔ3UTR together with either a synthetic miR-487b mimic or a nonspecific miR mimic (miR-433). The cells were then treated with LPS (100 ng/ml for 6 hr and analyzed for luciferase expression (). Data represent the . indicates samples with luciferase expression significantly different from control luciferase expression ().