Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa

Figure 1

Effect of PMNs and H₂O₂ on the adhesion of mucoid P. aeruginosa FRD1. The error bars indicate standard deviations. Control: control group; PMNs: FRD1 group treated with PMNs; 1 mM H₂O₂: FRD1 group treated with 1 mM H₂O₂; 2 mM H₂O₂: FRD1 group treated with 2 mM H₂O₂. (a) Quantification of biofilm formation using crystal violet staining. Cells were grown in Jensen’s medium for one day, in the presence of PMNs. PMNs increased the adhesion of P. aeruginosa FRD1. (b) The effect of PMNs on the number of viable cells is expressed in colony-forming units (CFUs). (c) Quantification of biofilm formation by crystal violet staining. H₂O₂ increased the adhesion of P. aeruginosa FRD1. (d) The effect of H₂O₂ on the number of viable cells is expressed in colony-forming units (CFUs). Data are presented as the mean ± SD ( in each treatment). compared to the control group.

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