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Figure 3: NRG treatment inhibits MP-induced BEAS-2B cells injury. (a) MTT analysis shows that NRG treatment has no significant effect on cell viability with different concentrations (). (b and c) Apoptosis of BEAS-2B cells was measured with FITC-annexin V/PI staining and flow cytometry in response to different treatments. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . , versus ctrl group; versus MPP group. (d–f) Inflammatory cytokines IL-6, IL-1β, and TNF-α expression in cell supernatant were measured with ELISA. (g) TGF-β expression in cell supernatant was measured with ELISA. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . versus ctrl group; versus MPP group. (h–k) Fibrotic correlation factor α-SMA, collagen I, and collagen III expression in cell were measured with western blot. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . versus ctrl group; versus MPP group.