Propofol Suppresses Proinflammatory Cytokine Production by Increasing ABCA1 Expression via Mediation by the Long Noncoding RNA LOC286367

Figure 2

LOC286367 and ABCA1were involved in LPS-induced inflammatory cytokines with propofol treatment in THP-1 macrophage cells. (a–c) THP-1 macrophages were incubated with propofol (prop) (0 μM), LPS (10 ng/ml), prop (0 μM) + LV-Mock + LPS (10 ng/ml), prop (50 μM) + LV-Mock + LPS (10 ng/ml), prop (0 μM) + LV-LOC286367 + LPS (10 ng/ml), and prop (50 μM) + LV-LOC286367 + LPS (10 ng/ml) in medium at 37°C for 24 h. (d–f) THP-1 macrophages were incubated with prop (0 μM), LPS (10 ng/ml), prop (0 μM) + negative control + LPS (10 ng/ml), prop (50 μM) + negative control + LPS (10 ng/ml), prop (0 μM) + ABCA1-siRNA + LPS (10 ng/ml), and prop (50 μM) + ABCA1-siRNA + LPS (10 ng/ml) in medium at 37°C for 24 h. All results are presented as mean ± SD of the three independent experiments; each being performed in triplicate. vs the control group.

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