Lepidium meyenii Walp Exhibits Anti-Inflammatory Activity against ConA-Induced Acute Hepatitis

Figure 5

Maca recruits more MDSCs to the liver and inhibits the infiltration of Kupffer cells and NKT cells in ConA-induced hepatitis. Hepatitis was induced by i.v. injection of ConA (15 mg/kg). Liver mononuclear cells were isolated from mice at 16 hours following ConA injection. (a) PE-CD11b⁺ FITC-Gr1⁺ MDSCs, (b) FITC-CD45⁺ APC-F4/80⁺ Kupffer cells, and (c) FITC-CD3⁺ APC-DX5⁺ NKT cells were stained and analyzed by a flow cytometer. Numbers in quadrants indicate the percentage of cells in each (a-B), (b-B), and (c-B). Proportions of (a-B) PE-CD11b⁺ FITC-Gr1⁺ MDSCs, (b-B) FITC-CD45⁺ APC-F4/80⁺ Kupffer cells, and (c-B) FITC-CD3⁺ APC-DX5⁺ NKT cells among total liver mononuclear cell population. The similar results are representative of three experiments. Data is expressed as the mean ± SD (). and vs. the ConA group. ConA, concanavalin A; Maca, Lepidium meyenii Walp; MDSCs, myeloid-derived suppressor cells; NKT cells, natural killer T cells.

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