Dynamic DNA Methylation Changes of Tbx21 and Rorc during Experimental Autoimmune Uveitis in Mice
Table 4
Methylation levels of Tbx21, Gata3, Rorc, and Foxp3 in the RPE-choroid complex of EAU mice at different time points.
Gene
CpG site
Naïve methylation level (%)
EAU 7th-day methylation level (%)
EAU 14th-day methylation level (%)
EAU 21st-day methylation level (%)
EAU 28th-day methylation level (%)
Tbx21
CpG_1
7.70 ± 1.89#
5.13 ± 1.31
1.50 ± 0.50#
6.25 ± 0.88#
7.00 ± 3.52#
CpG_3
13.58 ± 4.65
9.70 ± 5.83
5.42 ± 5.92
11.58 ± 6.87
6.58 ± 5.55
CpG_5
9.00 ± 3.41#
7.13 ± 3.01#
1.50 ± 1.22#
5.58 ± 1.28#
5.67 ± 0.98#
CpG_6, 7
4.75 ± 1.33
4.40 ± 1.98
2.58 ± 0.97
4.00 ± 1.26
2.58 ± 0.80
CpG_8
11.25 ± 2.50
8.25 ± 3.84
13.25 ± 2.93
12.50 ± 1.05
12.92 ± 2.13
Gata3
CpG_1
8.42 ± 3.20
1.50 ± 2.12
4.33 ± 2.25
4.40 ± 6.10
10.83 ± 5.23
CpG_2
25.30 ± 19.23
39.00 ± 13.30
32.17 ± 9.52
22.25 ± 5.76
21.25 ± 5.35
CpG_6, 7, 8
11.00 ± 2.28
23.67 ± 7.53
13.17 ± 7.78
21.25 ± 10.97
22.25 ± 12.32
CpG_9
1.67 ± 2.23
1.90 ± 3.05
2.83 ± 1.51
1.17 ± 0.52
0.83 ± 0.52
CpG_10, 11
12.50 ± 1.26
10.64 ± 5.23
32.17 ± 19.31
32.92 ± 20.45
33.25 ± 15.89
CpG_14
8.00 ± 4.72
8.08 ± 9.35
10.08 ± 4.43
2.92 ± 1.74
1.83 ± 1.51
CpG_16
7.50 ± 3.77
3.58 ± 5.61
5.83 ± 1.69
4.58 ± 1.43
2.00 ± 1.70
Rorc
CpG_1
13.13 ± 2.39
5.50 ± 6.60
4.40 ± 3.23
7.42 ± 3.01
6.20 ± 3.42
CpG_2
33.58 ± 10.93
37.83 ± 14.77
39.17 ± 7.13
16.28 ± 13.01
18.61 ± 14.38
CpG_3, 4
13.33 ± 4.12
10.83 ± 6.38
9.30 ± 5.38
14.00 ± 5.66
10.50 ± 0.71
CpG_5
26.40 ± 2.58#
23.17 ± 3.31#
13.00 ± 3.46#
24.33 ± 4.71#
22.00 ± 8.87
CpG_6
18.33 ± 7.74
20.17 ± 7.33
10.75 ± 4.67
11.42 ± 4.04
13.10 ± 3.50
Foxp3
CpG_2
74.33 ± 6.57
70.00 ± 9.25
79.83 ± 4.97
79.00 ± 7.55
86.50 ± 1.41
CpG_3
94.42 ± 6.79
99.83 ± 0.41
99.33 ± 1.63
91.25 ± 17.50
88.50 ± 16.26
CpG_5
78.90 ± 4.38
56.17 ± 9.09
72.58 ± 12.82
72.00 ± 11.31
59.00 ± 5.65
# indicates the data of the group showing a statistically significant (,, and ) difference compared with other groups among the five groups (one-way ANOVA followed by Bonferroni correction was used to compare the methylation levels in multiple groups).
