Heme Oxygenase-1 Reduces Sepsis-Induced Endoplasmic Reticulum Stress and Acute Lung Injury

Figure 2

Inhibition of endoplasmic reticulum stress could reduce lung injury in sepsis animals. Sprague-Dawley rats were injected intraperitoneally with 4-PBA (40 mg/kg) 30 min before CLP, and lung samples were collected 18 h following CLP. (a) Lung pathology was detected by using hematoxylin and eosin (H&E) staining. (b-c) Levels of lung injury score and water content in lung tissues. Lung injury score (b) evaluation was due to the pathology, and levels of the water content (c) in lung tissues were analyzed by using W/D method. (d) The expression of p-PERK protein was assayed by immunohistochemical technique. (e) Levels of endoplasmic reticulum stress-related genes, that is, PERK, eIF2-α, ATF4, and CHOP, were detected using real-time (RT) PCR method. PBA, 4-PBA. Data are shown as mean ± SEM (). and ; one-way ANOVA Tukey’s post hoc test.

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