Heme Oxygenase-1 Reduces Sepsis-Induced Endoplasmic Reticulum Stress and Acute Lung Injury

Figure 3

HO-1 activation could inhibit endoplasmic reticulum stress and reduced ER stress-related lung injury. Sprague-Dawley rats were intraperitoneally injected with saline, hemin (30 mg/kg) 24 h before operation with or without ZnPP (20 mg/kg) 12 h after hemin was given, then received celioectomy with or without CLP surgery. (a) Lung pathology was detected by using hematoxylin and eosin (H&E) staining. (b) Levels of lung injury score. Lung injury score evaluations were due to the pathology in lung H&E staining. (c–h) Levels of endoplasmic reticulum stress-related proteins, that is, HO-1, p-PERK, p-eIF2-α, ATF4, and CHOP, were detected using Western blot method, and gray analysis were performed according to the bands. p-PERK, phosphorylated PERK; p-eIF2-α, phosphorylated eIF2-α. Data are shown as mean ± SEM (). and ; one-way ANOVA Tukey’s post hoc test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)