Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses

Figure 4

MBL decreases PGN-induced phosphorylation of MAPKs in PMA-activated THP-1 cells. (a) PMA-activated THP-1 cells were stimulated with PGN (200 μg/ml), MBL (10 μg/ml) mixed with PGN, or anti-MBL pAb (10 μg/ml) mixed with MBL and PGN; then the cells were incubated at 37°C in a 5% (v/v) CO₂ environment for 8 h. All of the mixture was generated by preincubation for 2 h at room temperature. Cells were harvested, and Western blots were performed using p38 antibody (upper panel). β-Actin was used as loading control. The protein levels were quantitatively analyzed based on densitometry analysis (lower panel). (b) Cells were stimulated as described in (a). Western blots were performed using p-ERK (upper panel). The protein levels were quantitatively analyzed based on densitometry analysis (lower panel). (c) Western blots were performed using p-JNK (upper panel). The protein levels were quantitatively analyzed based on densitometry analysis (lower panel). Images are representative of three experiments. .

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