Research Article

Circulating Monocyte and Lymphocyte Populations in Healthy First-Degree Relatives of Type 2 Diabetic Patients at Fasting and during Short-Term Hyperinsulinemia

Figure 2

Effect of hyperinsulinemia on monocyte populations in the blood in FDR and in control subjects: (a) classical, intermediate, and nonclassical monocytes expressed as the percentage of positive cells related to CD45/14+; (b) monocyte populations expressing markers TLR2, CD163, CCR2, and CD36 expressed as the percentage of positive cells related to CD45/14+; (c) expression of markers TLR2, CD163, CCR2, and CD36 on monocyte subsets expressed as MFI ; , significant change during the euglycemic-hyperinsulinemic clamp; , significant difference between FDR and control subjects at baseline; striped bars—baseline preclamp levels in controls; grey bars—postclamp levels at the end (2 hours) of the clamp in controls; white bars—baseline preclamp levels in FDR; black bars—postclamp levels at the end (2 hours) of the clamp in FDR. FDR: first-degree relatives; MFI: mean fluorescence intensity.
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