Mediators of Inflammation / 2019 / Article / Fig 2

Research Article

Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro

Figure 2

(a) Preadipocytes SGBS were treated with indicated concentration of TB (200 μg/ml and 100 μg/ml) for 48 hrs. Cell proliferation was evaluated by MTT assay. The results are expressed as the percentage of the cell growth inhibition obtained by four experiments (preformed in triplicate). Bar represent the error standard of the percentage mean (). (b) Regulatory effects of TB on cell cycle progression in SGBS preadipocytes cultured in a medium containing 100 and 200 μg/ml TB for 24 h. The cells were stained with a PI solution and analyzed by flow cytometry. The acquired FACS data were analyzed by ModFit LT software.

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