Mediators of Inflammation / 2019 / Article / Fig 3

Research Article

Inflammatory Microenvironment and Adipogenic Differentiation in Obesity: The Inhibitory Effect of Theobromine in a Model of Human Obesity In Vitro

Figure 3

Inhibitory effect of TB on adipogenic differentiation in preadipocytes SGBS. (a) Inhibitory effect of TB on adipogenic differentiation in preadipocytes SGBS. The cells were cultured in a differentiation medium (DM) containing TB (100 μg/ml) added on day 8 from the start of the differentiation process. The cells were stained with Oil Red and were subject to a quantitative analysis of intracellular lipidic accumulation by an ELISA reader (λ = 490). The results are expressed as the percentage of the inhibition obtained by four experiments (preformed in triplicate). Bar represent the error standard of the percentage mean (). (b) Inhibitory effect of TB on adipogenic differentiation. The SGBS control (A) and the SGBS cultured in a differentiation medium (DM) containing TB (100 μg/ml) added on day 8 from the start of the differentiation process (B). Optical photography.
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