Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and the Bcl-2 Protein Family

Figure 2

pBAD intracellular protein levels in PBMO and CMBO in response to infection, AREG stimulation, and inhibition of EGFR. Isolated monocytes were infected with E. coli, extracellular bacteria were removed, and the cells were cultivated for 24 h in medium supplemented with antibiotics. 1 h prior to infection, AREG stimulation and EGFR inhibitor treatment were started and maintained during the entire cultivation period. Quantification was performed by using flow cytometry. (a) pBAD in PBMO and CBMO in response to infection and AREG stimulation. E. coli infection leads to BAD phosphorylation. Increase in pBAD is dose-dependently induced by AREG in both control and E. coli-infected PBMO and CBMO (). (b) Effect of EGFR inhibition on pBAD in PBMO and CBMO in response to infection and AREG stimulation. Inhibition of EGFR abolishes both AREG- and infection-induced increase in pBAD levels (). Data are shown as . Statistical significance was analyzed by using two-way ANOVA with Bonferroni’s multiple comparisons test. n.s.: not significant. .

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