Mediators of Inflammation / 2019 / Article / Fig 1

Research Article

PARP-1 Is Critical for Recruitment of Dendritic Cells to the Lung in a Mouse Model of Asthma but Dispensable for Their Differentiation and Function

Figure 1

Bone marrow progenitors were isolated from C57BL/6 mice and incubated with 20 ng/ml GM-CSF. (a) Cells were collected at days 0, 2, 4, 6, 7, 8, or 9. A portion of the cells at day 8 were stimulated with OVA for 24 h. Collected cells were processed for protein extraction followed by immunoblot analysis with antibodies to the indicated proteins. Blots were stripped of antibodies prior to probing with the next one. The two braces on the left represent two different gels of the same samples. (b) Bone marrow progenitors from WT or PARP-1−/− mice were isolated and processed as in (a). Protein extracts were subjected to immunoblot analysis with antibodies to the indicated proteins. For (a, b), signals were quantified and are shown as values under the respective blots.
(a)
(b)

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