Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic Stem/Progenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo

Figure 3

Neither conditioned media from PAC stimulated with atorvastatin nor treated cells affect the vascularization of subcutaneous Matrigel plugs. Example of an image of plug vascularization; blood flow is marked in red, borders of the plug in cyan (a). Gating for CD45⁺ cells and CD45^-CD31⁺ cells on DAPI-negative, viable single cells (b); plug vascularization assessed with ultrasonography with the PowerDoppler mode () (c); the percentage of infiltrating murine CD45⁺ cells measured with flow cytometry (d); the percentage of CD45^-CD31⁺ endothelial cells measured with flow cytometry (e) in plugs with control nonconditioned, conditioned, or conditioned media after the wash-out of atorvastatin (); plug vascularization (f); the percentage of infiltrating murine CD45⁺ cells (g) or CD45^-CD31⁺ endothelial cells (h) in plugs with PAC stimulated with 30 μmol/L atorvastatin for 48 hours ().

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