Research Article

Atorvastatin and Conditioned Media from Atorvastatin-Treated Human Hematopoietic Stem/Progenitor-Derived Cells Show Proangiogenic Activity In Vitro but Not In Vivo

Figure 5

Atorvastatin does not stimulate HMOX1 expression. 10 μmol/L sulforaphane induces higher levels of HMOX1 gene expression after 6 hours of treatment; , one-way ANOVA with Bonferroni post hoc test, (a). Neither HMOX1 overexpression, nor silencing, nor modulation of HMOX1 activity affects the paracrine angiogenic activity of PAC. Number of junctions in the Matrigel test in HAoEC cells stimulated with media from PAC transduced with lentiviral vectors encoding for either HMOX1 or shRNA HMOX1, (b); number of junctions in the Matrigel test in HAoEC cells stimulated with media from PAC with silenced HMOX1 stimulated with atorvastatin; vs. control cells treated with shSCR and DMSO, (c); number of junctions in the Matrigel test in HAoEC cells stimulated with control, nonconditioned media, or conditioned media from PAC stimulated with 10 μmol/L tin or cobalt protoporphyrin IX (SnPP or CoPP, respectively), (d).
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