Research Article
Inhibition of Synovial Macrophage Pyroptosis Alleviates Synovitis and Fibrosis in Knee Osteoarthritis
Table 1
Nucleotide sequences of primers used for RT-PCR amplification.
| | Target gene | Forward primer | Reverse primer |
| | Caspase1 | 5-TCGGAGAGTCGGAGCTGATGTT-3 | 5-CTCTGGGCAGGCAGCAAATTCT-3 | | NLRP3 | 5-CCAGACCTCCAAGACCACGACT-3 | 5-CCATCCGCAGCCAATGAACAGA-3 | | ASC | 5-GAGTCTGGAGCTGTGGCTACTG-3 | 5-ATGAGTGCTTGCCTGTGTTGGT-3 | | GSDMD | 5-TGCTTGCCGTACTCCATTCCATC-3 | 5-AGTTCTGAAGAGCCTGCCTCCA-3 | | IL-1β | 5-CTCATTGTGGCTGTGGAGAAG-3 | 5-ACACTAGCAGGTCGTCATCAT-3 | | IL-18 | 5-ACCGCAGTAATACGGAGCAT-3 | 5-TCTGGGATTCGTTGGCTGTT-3 | | HMGB1 | 5-AGTTCAAGGACCCCAATGCC-3 | 5-GTCATCCGCAGCAGTGTTGTT-3 | | TGF-β | 5-GACTCTCCACCTGCAAGACC-3 | 5-GGACTGGCGAGCCTTAGTTT-3 | | COL1A1 | 5-GTACATCAGCCCAAACCCCA-3 | 5-CAGGATCGGAACCTTCGCTT-3 | | PLOD2 | 5-AAGACGCTGCGATCAGAGAT-3 | 5-AGTGGGGGAGTCTTTTTCCC-3 | | TIMP1 | 5-TGTGGGAAATGCCACAGGTT-3 | 5-TTCCGTTCCTTAAACGGCCC-3 | | GAPDH | 5-GGCCTTCCGTGTTCCTACC-3 | 5-ACTCGACACCTGCCCTCA-3 |
|
|
