Mycobacterium tuberculosis Requires Cholesterol Oxidase to Disrupt TLR2 Signalling in Human Macrophages

Figure 5

IRAK4 and TRAF6 protein levels in macrophages treated with PMA, IL-1β, or LTA. The macrophages were cultured for 24 hours in the presence of PMA, lipoteichoic acid (LTA), or human recombinant interleukin 1β (hrIL-1β). Next, the cells were lysed, and the IRAK4, p-IRAK4, and TRAF6 protein levels were assessed using the immunoblot-ECL method. Representative immunoblots of the IRAK4 and TRAF6 protein levels are shown. The bands were quantified by densitometric analysis. The data are presented as the optical density intensity of the area under each band’s peak (ODI)±SEM from 2 independent experiments.