Mycobacterium tuberculosis Requires Cholesterol Oxidase to Disrupt TLR2 Signalling in Human Macrophages

Figure 6

The IRAK4 and TRAF6 mRNA levels in Mtb-infected macrophages. The macrophages were infected with the wild-type, ΔchoD, or ΔchoD-choD strains for 0.5 to 24 hours, and the total RNA was isolated. The IRAK4 and TRAF6 mRNA level was measured using qRT-PCR. The data are presented as the from 5 independent experiments. The solid line marks the level of gene expression in control cells (noninfected macrophages). RQ represents the fold change in the gene expression in the infected macrophages compared to the noninfected macrophages, calculated using the ABI 7900HT RQ Manager Software v1.2 and DataAssist Software v3.01 (Thermo Fisher Scientific). Macrophages infected with wild-type, ΔchoD, or ΔchoD-choD Mtb vs. noninfected macrophages, ; ^#Macrophages infected with ΔchoD Mtb vs. macrophages infected with wild-type Mtb, (-test).