IFN Regulatory Factor 1 Mediates Macrophage Pyroptosis Induced by Oxidized Low-Density Lipoprotein in Patients with Acute Coronary Syndrome

Figure 6

Effects of IRF-1 on the NLRP3-ASC inflammasome in macrophages. (a) Macrophages from ACS patients were aliquoted into four groups and treated with Dil-ox-LDL. Relative mRNA and protein levels of NLRP3 and ASC were measured by RT-PCR and western blot. indicates vs ACS group (). indicates vs ACS and IRF-1 group (). # indicates vs ACS group (^#). (b) Macrophages were transfected with pGC-FU-IRF-1 for 36 h, and then, cells were transfected with nontargeting siRNA and siRNA specific for NLRP3 and ASC. Caspase-1 activity was detected by Ac-YVAD-pNA. indicates vs ACS group (). # indicates vs ACS group (^#). (c) Macrophages were transfected with NLRP3 siRNA specific or ASC siRNA after transfection with pGC-FU-IRF-1 as described. Protein of caspase-1 p10, IL-1β p17, IL-18, and GSDMD-N were measured by western blot. indicates vs ACS group (). # indicates vs ACS group (^#).

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