Research Article

Interleukin-12p35 Deficiency Reverses the Th1/Th2 Imbalance, Aggravates the Th17/Treg Imbalance, and Ameliorates Atherosclerosis in ApoE-/- Mice

Figure 3

Effect of IL-12p35 deficiency on the activity of Th1 and Th2. (a) The frequency of Th1 and Th2 in ApoE-/- mice and ApoE-/- IL-12p35-/- mice was measured by flow cytometry, for each group. (b) The serum IFN-γ and IL-4 levels were detected by ELISA; for each group. (c, d) The mRNA levels of T-bet, IFN-γ, GATA3, and IL-4 in the spleens and aortas of these two groups were analyzed by RT-qPCR. ApoE KO = ApoE-/-, DKO = ApoE-/- IL-12p35-/-; for each group. vs. the ApoE-/- group.