CD36-Mediated Lipid Accumulation and Activation of NLRP3 Inflammasome Lead to Podocyte Injury in Obesity-Related Glomerulopathy

<div>Effects of inhibiting CD36 on leptin-induced activation of NLRP3 inflammasome and IL-1<i>β</i> secretion of podocyte. (a) Differentiated podocytes were incubated in medium, medium containing 15 ng/mL leptin, or medium containing both 15 ng/mL leptin and 50 <i>μ</i>M SSO, respectively. (b) Differentiated podocytes were transiently transfected with 50 pmol control siRNA or CD36 siRNA, respectively. After 24 h, cells were incubated in medium or medium containing 15 ng/mL leptin as indicated, respectively. (a) and (b) The relative mRNA and protein expression levels of NLRP3, pro-caspase-1, and IL-1<i>β</i> of podocytes were measured by real-time quantitative PCR and Western blot assay. 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Mediators of Inflammation

fig7

Figure 7

Figure 7: CD36-Mediated Lipid Accumulation and Activation of NLRP3 Inflammasome Lead to Podocyte Injury in Obesity-Related Glomerulopathy 