Dihydrotanshinone I Alleviates Crystalline Silica-Induced Pulmonary Inflammation by Regulation of the Th Immune Response and Inhibition of STAT1/STAT3

Figure 3

DHI modulated Th1/Th2 immune responses in a mouse model of CS-induced inflammation. Percentage of CD4+IFN-γ+ Th1 cells in the HLN (a, c) and spleen (b, d) was detected by flow cytometry ( to 6 per group). (e) ELISA analysis of IFN-γ levels in the BALF ( per group). (f, g) Th1 nuclear transcription factor T-bet expression levels in mouse lung and spleen were assayed by qRT-PCR ( to 6 per group). (h, i) ELISA analysis of IL-4 and IL-13 levels in the BALF ( per group). (j) Th2 nuclear transcription factor GATA3 expression levels in mouse lung were assayed by qRT-PCR ( to 6 per group). Data are presented as the . ; . All experiments were performed in triplicate.

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