Research Article

Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells

Figure 5

Oxidative stress regulates DEP-induced EGFR activation in THP-1 cells. THP-1 cells were pretreated with 10 mM NAC for 2 h, prior to 100 μg/ml DEP treatment for 4 h. Then, the treated THP-1 cells were lysed, and the supernatants of cell lysates were subjected to SDS-PAGE and immunoblotting using phospho and pan antibodies against EGFR. Data shown are representative of three separate experiments.