Research Article

The cAMP Pathway Amplifies Early MyD88-Dependent and Type I Interferon-Independent LPS-Induced Interleukin-10 Expression in Mouse Macrophages

Figure 4

Isoproterenol inhibits LPS-stimulated type I IFN secretion. RAW264.7 macrophages were incubated with LPS (10 ng/ml)±isoproterenol (Iso, 1 μM) for 1 h. The LPS-containing medium was then removed, and the conditioned medium was collected following 3 h without further stimulus. (a) IL-10 secretion from the macrophages was measured by ELISA. (b) Type I IFN level in the media was measured by a luciferase reporter ISRE-L929 cell line assay. Data expressed as the () of values normalized against Renilla luciferase activity and divided by 10,000. for cells costimulated with LPS+isoproterenol compared to cells stimulated with LPS alone. The experiment was carried out 5 times with similar results.
(a)
(b)