Figure 1: C2C12 myogenic differentiation. Expression of MURC, myogenin and MyHC mRNA (a) and protein (b) from C2C12 cells at the time indicated during myogenesis in media containing either 10% fetal bovine serum (FBS) or 3% horse serum (HS) was examined (n = 3). and v.s 10% FBS at day 0, #p < 0.05 v.s 3% HS at day 0, p < 0.05 v.s 10% FBS group at the same day. (c) Morphology of cells at the indicated day was stained with DAPI and examined by fluorescence microscopy.