Figure 3: IL-4 boosts insulin signaling in C2C12 myoblasts. Cells were transferred to serum free medium for 3 hr, followed by exposure to IL-4 and/or insulin for 30 min. (a) Cell lysate were harvested at indicated time, and p-Akt and p-GSK-3β were analyzed (n = 3). (b) Cells were transferred to KRPH buffer for 3 hr, and cellular glucose uptake was measured as described in Methods (n = 3). v.s 0 min, #p < 0.05 v.s INS group at the same time point.