miR-221 Alleviates the Ox-LDL-Induced Macrophage Inflammatory Response via the Inhibition of DNMT3b-Mediated NCoR Promoter Methylation

Figure 1

miR-221 suppressed the ox-LDL-induced macrophage inflammatory response. (a–d) The expression of miR-221, IL-6, and TNFα and activity of NF-κB were detected by qPCR after THP-1-derived macrophages were treated with ox-LDL at the indicated times (). , vs. the 0 h group; ^{^}, vs. the 24 h group. (e–g) NF-κB activity and IL-6 and TNF-α expression were evaluated by a luciferase assay, qPCR, and flow cytometry (FCM) after THP-1 cells were transfected with miR-221 mimic for 48 h, differentiated into macrophages, and incubated with ox-LDL for another 24 h (). , vs. the NC group; ^{^}, vs. the ox-LDL/NC group; ^#, vs. the miR-221 mimic group.

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