Mediators of Inflammation / 2019 / Article / Fig 2

Research Article

miR-221 Alleviates the Ox-LDL-Induced Macrophage Inflammatory Response via the Inhibition of DNMT3b-Mediated NCoR Promoter Methylation

Figure 2

DNMT3b is a direct target of miR-221. (a) Predicted alignment between the miR-221 sequence and the wild-type (WT) and mutated (MUT) 3UTRs of DNMT3b. (b) A luciferase assay evaluated the reporter activity of the DNMT3b WT and MUT 3UTRs in 293T cells (). , vs. the NC group. (c, d) Western blot detected the protein expression of DNMT3b and NCoR after THP-1-derived macrophages were transfected with miR-221 mimic or inhibitor for 48 h (). , vs. the NC group.

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