miR-221 Alleviates the Ox-LDL-Induced Macrophage Inflammatory Response via the Inhibition of DNMT3b-Mediated NCoR Promoter Methylation
DNMT3b is involved in the ox-LDL-mediated promoter methylation of NCoR. (a) Schematic of the CpG island in the promoter region of NCoR. (b) MSP was used to analyze DNA methylation in the NCoR promoter region (). (c) Western blot analysis of the protein expression of DNMT3b after THP-1-derived macrophages were treated with ox-LDL ()., vs. the NC group; (d, e) the protein and mRNA expression of NCoR was evaluated by qPCR and western blotting after THP-1-derived macrophages were stimulated with ox-LDL in the presence or absence of 5-AZA ()., vs. the NC group; ^, vs. the ox-LDL group.
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