Research Article

Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

Figure 6

Lats2-underexpressing BMSCs inhibit early pulmonary fibrosis. (a) Picrosirius red staining of collagen fibers (red staining) in the lung tissues at 14 days after LPS challenge. (Picrosirius red staining; magnification, ×200; scale μm). (b) Quantification of collagen area in Picrosirius red stained-lung sections at 14 days after LPS challenge. (c) Immunohistochemistry for α-SMA (dark brown staining) in the lung tissues at 14 days after LPS challenge (magnification, ×400; scale μm). (d) Quantification of mean optical density (MOD) for α-SMA in the lung tissues at 14 days after LPS challenge. (e) Immunohistochemistry for TGF-β1 (dark brown staining) in the lung tissues at 14 days after LPS challenge (magnification, ×400; scale μm). (f) Quantification of MOD for TGF-β1 in the lung tissues at 14 days after LPS challenge. (g) Hydroxyproline content of lung homogenates at 14 days after LPS challenge. (h) The mRNA expression of Collagen Type I Alpha 1 (Col1α1) in the lung tissue at 7 days after LPS challenge. GAPDH was used as the internal reference gene. (i) The mRNA expression of Collagen Type III Alpha 1 (Col3α1) in the lung tissue at 7 days after LPS challenge. GAPDH was used as the internal reference gene. Data are presented as the deviation (). .
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