Curcumin Attenuates Asthmatic Airway Inflammation and Mucus Hypersecretion Involving a PPARγ-Dependent NF-κB Signaling Pathway In Vivo and In Vitro
PPARγ expression was inhibited after transfection of the lung with shRNA-PPARγ. Mice were transfected with shRNA-PPARγ or shRNA-scrambled by intratracheal injection. Twenty days after transfection, the expression of PPARγ was measured. (a) After 20 days of transfection, mice were sacrificed and their left lower lungs were fixed. The tissue sections were then stained with H&E. The figure demonstrates a representative view (×200) from each group. (b) qPCR was used to analyze the mRNA expression of PPARγ. (c) Western blotting was performed to evaluate the protein expression of PPARγ. Each bar represents the of 10 mice. compared with control.