miR-23b Negatively Regulates Sepsis-Induced Inflammatory Responses by Targeting ADAM10 in Human THP-1 Monocytes

Figure 1

Analysis of miR-23b, ADAM10, inflammatory cytokines, and apoptosis in patients with sepsis and LPS-stimulated THP-1 cells. Peripheral blood mononuclear cells (PBMCs) and serum were extracted from the blood samples of sepsis patients () and healthy controls (). The expression of miR-23b (a) and ADAM10 (b) in PBMCs was detected by qRT-PCR. The serum concentrations of IL-1β (c), IL-6 (d), and TNF-α (e) were measured by ELISA. THP-1 cells were treated with LPS (500 ng/mL) for different time periods (6, 12, and 24 hours). miR-23b and ADAM10 mRNA expression was detected by qRT-PCR analysis, and the inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured by ELISA (f, g). ADAM10 protein was also measured by western blotting (h). Evaluation of apoptosis of THP-1 cells treated with PBS or LPS (500 ng/mL) for 12 h was performed using flow cytometric analysis of Annexin V-FITC/PI staining (i). Each experiment was independently repeated at least three times. , , and .

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