miR-23b Negatively Regulates Sepsis-Induced Inflammatory Responses by Targeting ADAM10 in Human THP-1 Monocytes

Figure 5

miR-23b alleviates the inflammatory response and apoptosis in THP-1 cells upon LPS stimulation by directly targeting ADAM10. The transfection efficiency of LV3-ADAM10-shRNA in THP-1 cells was evaluated by qRT-PCR and western blot (a, b). The miR-23b mimics and/or ADAM10 knockdown significantly decreased ADAM10 protein production (c). The apoptosis of THP-1 cells detected by the Annexin V-FITC/PI (green/red) double staining assay. The percentage of cells in early and late stages of apoptosis was obtained by analysis of the cell images (, at least 3 experiments) (d, e). Knockdown of ADAM10 significantly decreased the LPS-stimulated production of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and apoptosis rate in THP-1 cells (e–h). Combination of miR-23b mimics and ADAM10 knockdown did not significantly decrease the LPS-stimulated inflammatory response and apoptosis of THP-1 cells compared with miR-23b mimics or ADAM10 knockdown.

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