IGF-1R Inhibition Suppresses Cell Proliferation and Increases Radiosensitivity in Nasopharyngeal Carcinoma Cells

Figure 4

Linsitinib with or without IR induces cell cycle arrest and apoptosis in NPC cells. (a) CNE-2 and SUNE-1 cells were treated with linsitinib (Lin., 0.4 μM) and/or IR (4 Gy) for 24 h. Then, cells were harvested and stained with propidium iodide (PI) staining, and cell cycle was determined by flow cytometry. (b) CNE-2 and SUNE-1 cells were treated as cell apoptosis assay and stained with annexin V-FITC/PI. The quantification of the percentage of apoptotic cells includes early apoptotic (bottom right quarter) and late apoptotic (top right) cells. (c) The statistical graph of (b). Each experiment was repeated at least three times.

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