Figure 5: Histological characteristics from the therapeutic regimen. Representative H&E and Safranin-O-stained slides from the PBS group (a–c) and from the high-dose RvE1 group after RA development (d, f). In this strategy, all mice showed clinical signs of arthritis at the start of treatment. As expected, CIA was very pronounced in the absence of treatment (a–c). In the Safranin-O-stained slides, proteoglycan depletion was noted (c). High dosage of RvE1 did not reduce inflammation, cartilage erosion, bone destruction, and proteoglycan depletion (d–f) in the ankle joints of CIA mice. Original magnification of 10x and 20x.